Synthesis, Characterization, Antibacterial and Antifungal studies of Silver Nanoparticles from Ocimum gratissimum L.

Abstract

A vital need in the field of nanotechnology is the development of reliable and eco-friendly processes for synthesis of
metallic nanoparticles. This present study revealed the green synthesis of Silver nanoparticles from the leaf extract of
Ocimum gratissimum L. The nanoparticles synthesis was confirmed by colour change from yellow to dark brown. The
Surface Plasmon absorption bands at different time intervals 10, 30 and 60 min were recorded at 421 nm, 428 nm and 439
nm respectively. Further the nanoparticles were characterized by Scanning Electron Microscopy, Energy dispersive x-ray
analysis and Fourier Transform Infra Red spectroscopy. The antibacterial activity of Silver nanoparticles was evaluated
against Escherichia coli and Staphylococcus aureus. The antifungal activity of Silver nanoparticles was evaluated against
Aspergillus niger. Extracellular synthesis of Silver nanoparticles using Ocimum gratissimum leaf extract is conventional,
eco-friendly and cost effective.