METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN, FENOFIBRATE AND FOLIC ACID IN PHARMACEUTICAL DOSAGE FORM

K. Shivani; G.Tulja Rani

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN, FENOFIBRATE AND FOLIC ACID IN PHARMACEUTICAL DOSAGE FORM

Authors

K. Shivani Department Of Pharmaceutical Analysis and Quality Assurance, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad, Telangana State, India.
G.Tulja Rani Department Of Pharmaceutical Analysis and Quality Assurance, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad, Telangana State, India.

Keywords:

RP-HPLC, Atorvastatin, Fenofibrate and Folic acid, Validation

Abstract

A simple, rapid, accurate and precise isocratic reverse phase high performance liquid chromatography (RP-HPLC) method
has been developed for simultaneous estimation of atorvastatin, fenofibrate and folic acid in combined dosage form by
using C18 column. Mobile phase consisting of a 40 volumes of mixed phosphate buffer (pH 6.0) : 60 volumes of acetonitrile
and detection was carried out at 235 nm and the retention times of atorvastatin, fenofibrate and folic acid were found to be
4.407, 3.393and 7.450 min respectively. The developed method was validated as per ICH guideline for specificity, linearity,
accuracy, and precision and system suitability. The new RP-HPLC method was successfully applied to marketed
formulation without any interference from excipients.

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Published

2015-04-29

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How to Cite

K. Shivani, and G.Tulja Rani. “METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN, FENOFIBRATE AND FOLIC ACID IN PHARMACEUTICAL DOSAGE FORM”. International Journal of Pharmaceutics and Drug Analysis, vol. 3, no. 4, Apr. 2015, pp. 140-9, https://www.ijpda.org/index.php/journal/article/view/147.