RECENT TRENDS IN ION-EXCHANGE CHROMATOGRAPHY

Authors

  • Omprakash G. Bhusnure Channabasweshwar Pharmacy College, Dept of Quality Assurance, Latur(MS), India
  • Supriya N Mali Channabasweshwar Pharmacy College, Dept of Pharmacutics, Latur(MS), India

Keywords:

Ion Exchange Chromatograph, Ion-Exchange Phases, Resin selection, Factors affecting, Application

Abstract

Ion exchange chromatography involves the separation of ionizable molecules based on their total charge. This technique enables the separation of similar types of molecules that would be difficult to separate by other techniques because the charge carried by the molecule of interest can be readily manipulated by changing buffer pH. Ion exchange chromatography is commonly used to separate charged biological molecules such as proteins, peptides, amino acids, or nucleotides. The amino acids that make up proteins are zwitterionic compounds that contain both positively and negatively charged chemical groups. Depending on the pH of their environment, proteins may carry a net positive charge, a net negative charge, orno charge. Ion-exchange chromatography is a relatively mature area of chromatographic separation yet advances in this technique continue unabated. This review highlight the basic principle, techniques, selection criteria of resin, factors affecting, applications of in pharmaceutical industry and also provides a summary of the latest in new ion-exchange phases for ion chromatography. It starts by focusing on general aspects of phase design and then reviews anion-exchange and cation-exchange columns introduced in the past few years.

Downloads

Download data is not yet available.

References

T. Stevens, U. S. Patent 4,351,909, May 21, 1981.

D. Jensen, J. Weiss, M.A. Rey, and C.A. Pohl, J Chromatogr. 640(1–2), 65–71 (1993).

J. Riviello, M. Rey, J. Jagodzinski, and C.A. Pohl, U. S. Patent 5,865,994, February 2, 1999.

P. Kolla, J. Köhler, and G. Schomburg, Chromatographia 23, 465 (1987).

C. Pohl and C. Saini, J Chromatogr. A 1213(1), 37–44 (2008).

C.A. Pohl and C. Saini, U. S. Patent 7,291,395, November 6, 2007.

Pharmaceutical analysis, instrumental methods by Dr.A.V.Kasture.

Instrumental methods of chemical analysis by Gurudeep Chatwal and Sham K. Anand.

Text book of pharmaceutical analysis by Dr.S. Ravisankar.

Published

2015-12-29
Statistics
483 Views | 297 Downloads
Citatons

How to Cite

Omprakash G. Bhusnure, and Supriya N Mali. “RECENT TRENDS IN ION-EXCHANGE CHROMATOGRAPHY”. International Journal of Pharmaceutics and Drug Analysis, vol. 3, no. 12, Dec. 2015, pp. 403-16, https://www.ijpda.org/index.php/journal/article/view/187.

Issue

Section

Review Articles
Share |