RECENT TRENDS IN ION-EXCHANGE CHROMATOGRAPHY
Keywords:
Ion Exchange Chromatograph, Ion-Exchange Phases, Resin selection, Factors affecting, ApplicationAbstract
Ion exchange chromatography involves the separation of ionizable molecules based on their total charge. This technique enables the separation of similar types of molecules that would be difficult to separate by other techniques because the charge carried by the molecule of interest can be readily manipulated by changing buffer pH. Ion exchange chromatography is commonly used to separate charged biological molecules such as proteins, peptides, amino acids, or nucleotides. The amino acids that make up proteins are zwitterionic compounds that contain both positively and negatively charged chemical groups. Depending on the pH of their environment, proteins may carry a net positive charge, a net negative charge, orno charge. Ion-exchange chromatography is a relatively mature area of chromatographic separation yet advances in this technique continue unabated. This review highlight the basic principle, techniques, selection criteria of resin, factors affecting, applications of in pharmaceutical industry and also provides a summary of the latest in new ion-exchange phases for ion chromatography. It starts by focusing on general aspects of phase design and then reviews anion-exchange and cation-exchange columns introduced in the past few years.
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