DETERMINATION OF GLIMEPIRIDE & EZETIMIBE IN COMBINE TABLET DOSAGE FORM BY RATIO DERIVATIVE SPECTROSCOPY AND Q-ABSORBANCE METHOD

Abstract

Glimepiride is antidiabetic drug and Ezetimibe is cholesterol absorption inhibitor.The combination of this two drug is used for the treatment of hypercholesterolemia.Two new ,simple,accurate and precise uv spectrophotometric methods have been developed amd validated for the simultaneous determination of Glimepiride (GLM) and Ezetimibe (EZE) in their combine dosage forms. The first method is ratio derivative spectroscopy method (Method A) in which ratio derivative amplitudes were measured at selected wavelengths. The amplitudes at 212.4 nm and 254.2 nm in the ratio derivative spectra were selected to determine GLM and EZE, respectively. The second method is Q-absorbance method. For Q-absorbance method the absorbances of the standard solutions were taken at two wavelengths 228.0 nm (?max of glimepiride) and 236.0 nm (Isobestic point), in methanol. Beer’s law is obeyed in the concentration ranges of 6-16 ?g/ml for  GLM and EZE  in methanol for both the methods. Both methods were validated statistically and recovery studies carry out.The suitability of this method for the quantitative determination of Glimepiride and Ezetimibe was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Glimepiride and Ezetimibe in combination. The results of analysis have been validated statistically and by recovery studies.The validation study is statistically significant as all statistical parameter are within the acceptance range ( % RSD < 2.0  and S.D. < 2.0) for both accuracy and precision.