DEVELOPMENT AND VALIDATION OF RP HPLC METHOD FOR THE ESTIMATION OF ALFUZOSIN IN TABLET FORMULATION
Keywords:
Alfuzosin, Method Validation, HPLC, C8 ColumnAbstract
A new simple, rapid, precise and accurate assay method was developed forestimation of Alfuzosin in pure form and tablet form. The analyte was separated on a C8 (Primesil) column (5 µm, 4.6mm* 250 mm).The mobile phase was Acetonitrile:0.05 % Ortho Phosphoric Acid(40:60, v/v) after adjusting pH to 3.2 this pumped at 0.7 mL/min flow rate it passes all the parameters satisfactorily. The UV detector was operated at 242 nm for the determination of all the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 10-50 µg/ml for Alfuzosinwith a R2of value0.997. The optimized methods proved to be specific, robust and accurate for the quality control of drugs in bulk drug and pharmaceutical formulations.
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