DEVELOPMENT AND VALIDATION OF DUAL WAVELENGTH UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME AND DICLOXACILLIN SODIUM IN PHARMACEUTICAL DOSAGE FORM
Keywords:
Cefixime, Dicloxacillin sodium, Dual wavelength method, UV spectrophotometric method, ValidationAbstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical dual wavelength method for the simultaneous determination of Cefixime and Dicloxacillin sodium in tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. Cefixime was determined directly at 271.8 nm in methanol. The wavelengths selected for determination of dicloxacillin sodium were 271.8 nm and 218.4 nm in methanol. Regression analysis of Beer’s plots showed good correlation in concentration range of 3?16 ?g/ml for both the drugs. The method was successfully applied for the determination of these two drugs in tablet dosage form. No interference was observed from excipients present in the tablet. The suitability of this method for the quantitative determination of Cefixime and Dicloxacillin sodium was proved by validation. The proposed method was found to be simple and sensitive for the routine analysis of these two drugs in tablet dosage form. The results of analysis have been validated statistically and by recovery studies.
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