Isolation and characterization of flavonoid from ethanolic extract of leaves of Naravelia zeylanica

Abstract

In this study the phytochemical analysis of leaves of Naravelia zeylanica (Ranunculaceae) plant in various ether extracts were taken up. The ether layer II was taken for study as it was screened positively for the flavonoid type of compound. The extract was chromatographed by preparative-TLC using ethanol: ethyl acetate 8.5:1.5 as the eluent.One of the flavonoid types of compound was isolated by the chromatographic method. Then, the compound was subjected to the routine chemical and spectroscopic analyses. The compound was found to be8-hydroxy-2(4-hydroxy-5methoxyphenyl)-7-(tetrahydro-3,4,5-trihydroxy-6-(hydroxymethyl)-2H-pyran-2yloxy)-4H-chromen-4-one.