CARDIOPROTECTIVE EFFECT OF SEENTHIL CHOORANAM IN ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN WISTAR RATS

Abstract

Background: Oxidative stress-mediated myocardial damage is a key factor in the development of cardiovascular diseases. Seenthilchooranam, a classical Siddha formulation, is believed to possess antioxidant and cardioprotective properties. The present study aimed to evaluate the cardioprotective activity of Seenthilchooranam against isoproterenol-induced myocardial toxicity in wistar albino rats.

Aim: To evaluate the cardio protective activity of Seenthilchooranam Against isoproterenol -induced myocardial injury in wistar rats by assessing antioxidant status, heart/body weight ratio, biochemical markers and histopathological changes.

Materials and Methods: Wistar albino rats were allocated into five groups[n=6]: normal, isoproterenol control, vitamin E- treated standard group and two treatment groups receiving low and high doses of SC. Treatments were given orally for 28 days, followed by ISO injections for two consecutive days to induce myocardial injury. Serum cardiac marker enzymes [CK-MB, LDH, AST], heart weight/body weight ratio and cardiac antioxidant parameters such as malondialdehyde [MDA], superoxide dismutase [SOD], catalase [CAT] and reduced glutathione [GSH] were evaluated. Cardiac tissues were further examined histologically. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparison tests.

Result: Isoproterenol challenge resulted in significant damage in the myocardium, characterized by increased cardiac enzyme release, elevated lipid peroxidation, and decreased the levels of antioxidant enzymes and structural abnormalities in heart tissue. Treatment with Seenthilchooranam significantly improved these biochemical and tissue parameters in a dose-dependent manner with the higher dose showing effects comparable to the standard drug. Histopathological findings supported the biochemical results showing improved myocardial architecture and reduced cellular degeneration.

Conclusion: Seenthilchooranam demonstrated significant cardioprotective activity against isoproterenol- induced myocardial injury, possibly through antioxidant, membrane-stabilizing and free-radical scavenging mechanisms. These results support the potential role of Seenthilchooranam as a cardioprotective agent in oxidative stress-related cardiac disorders.