STANDARDIZATION OF SIDDHA POLYHERBAL FORMULATION “INJI RASAYANAM”

Abstract

Inji Rasayanam is a classical Siddha polyherbal formulation indicated for the management of Soothagavaayu, which is referred to as Polycystic Ovarian Disease (PCOD). Although its traditional efficacy is well documented, the lack of standardized quality control parameters poses a challenge to its global acceptance. The present study aimed to establish the standardization profile of Inji Rasayanam through physicochemical evaluation, phytochemical analysis, HPTLC fingerprinting, microbial load assessment, and aflatoxin analysis. The physicochemical parameters, such as total ash value, acid-insoluble ash, loss on drying, alcohol extractive value, and water extractive value, were analyzed according to standard protocols. Phytochemical screening revealed the presence of carbohydrates. The HPTLC fingerprinting profile showed 10 peaks at 254 nm and 6 peaks at 366 nm, indicating the presence of multiple phytoconstituents. Microbial load and aflatoxin levels were found to be within WHO permissible limits.These observations contribute to the scientific framework and confirm the quality and safety of Inji Rasayanam.