INSTRUMENTAL STANDARDIZATION OF SIDDHA FORMULATION CHITHIRAMOOLA RASAYANAM USING FTIR, SEM AND HEAVY METAL ANALYSIS

Abstract

Objective: The present study aims to standardize a Siddha Polyherbal formulation (Chithiramoola Rasayanam) using modern analytical techniques to ensure its quality, safety, and efficacy.

Methods: The formulation was prepared using purified raw drugs including Chithiramoolam (Plumbago zeylanica), Nilappanai (Curculigoorchioides), Thannirvittan (Asparagus racemosus), Parangichakkai (Smilax china), Serangkottai (Semecarpus anacardium), along with honey, ghee, and sugar, following classical Siddha procedures. The prepared drug was subjected to Fourier Transform Infrared Spectroscopy (FTIR) for identification of functional groups, Scanning Electron Microscopy (SEM) for surface morphology and particle size analysis, and Heavy Metal Analysis to assess safety by detecting toxic metals such as lead, cadmium, arsenic, and mercury.

Results: FTIR analysis revealed characteristic peaks corresponding to hydroxyl, aliphatic, carbonyl, and ether groups indicating the presence of phytoconstituents such as phenols, flavonoids, and glycosides. SEM analysis showed irregular, agglomerated particles with rough surface morphology, suggesting effective trituration and enhanced surface area. Heavy metal analysis confirmed that all toxic metals were within WHO permissible limits.

Conclusion: The findings confirm that the formulation possesses significant physicochemical characteristics and meets safety standards. This study provides scientific validation and supports the standardization of the Siddha formulation.